Pierce™ Protein G IgG Binding Buffer 1 L

Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Protein G IgG Binding Buffer is formulated specifically for antibody purification using Protein G.

Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:

• Ready to use—carefully formulated and prefiltered 1X solutions

• Three binding buffers—optimized for Protein A, Protein G or Protein A/G

• Two elution buffer options—acidic (pH 2.8) or high salt (near-neutral)

• Versatile—elution buffers are appropriate for antibody-antigen affinity purification

Products available for each antibody purification method:

• Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA

• Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA

• Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide

• IgG Elution Buffer: pH 2.8; amine-based

• Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide

• Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)