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GENEART GEN CLEAV DET KIT

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The GeneArt Genomic Cleavage Detection Kit is a fast, T7 endonuclease I based method to quantify how well your genome editing protocol causes insertions and deletions (indels) in the genome of your cell line. This is the quickest way to quantify and validate the best CRISPR-Cas9 gRNA, or the best TAL effector nuclease, for your cell engineering experiment. The GeneArt Genomic Cleavage Detection Kit provides a convenient, quick, and complete solution.

Using this T7 endonuclease I (T7EI) based method, you can quickly and confidently measure your on-target genome editing efficiency generated by non-homologous end joining (NHEJ) activity. The advantages of the GeneArt Genomic Cleavage Detection Kit are:

• Minimal hands-on time — PCR amplify from cell lysate, no DNA purification required
• Short protocol — four hours from cell harvest to quantified results
• Just add PCR primers — everything needed in one box. No separate PCR master mixes or DNA extraction kits to buy.
• Quantify editing efficiency directly from your gel — gel band density directly correlates to on-target indel formation

Obtain results the same day without waiting for sequencing results or performing complicated sequencing analysis.
For Research Use Only. Not for use in diagnostic procedures.

Catalog No - A24372
HSN - 38229090
₹ 39,303.00 ₹ 39,303.00  ​

₹ 39,303.00 / Units
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  • Industry
Terms and Conditions
Packing, Forwarding, Freight & Insurance Charges Extra. 
Installation(If applicable) charges Extra.
Validation(If applicable) charges Extra.
Payment 100% advance.
Shipping Within 3-5 working days.
​Brand: Invitrogen

               


                  Product Details

The GeneArt Genomic Cleavage Detection Kit is a fast, T7 endonuclease I based method to quantify how well your genome editing protocol causes insertions and deletions (indels) in the genome of your cell line. This is the quickest way to quantify and validate the best CRISPR-Cas9 gRNA, or the best TAL effector nuclease, for your cell engineering experiment. The GeneArt Genomic Cleavage Detection Kit provides a convenient, quick, and complete solution.


Using this T7 endonuclease I (T7EI) based method, you can quickly and confidently measure your on-target genome editing efficiency generated by non-homologous end joining (NHEJ) activity. The advantages of the GeneArt Genomic Cleavage Detection Kit are:


• Minimal hands-on time — PCR amplify from cell lysate, no DNA purification required

• Short protocol — four hours from cell harvest to quantified results

• Just add PCR primers — everything needed in one box. No separate PCR master mixes or DNA extraction kits to buy.

• Quantify editing efficiency directly from your gel — gel band density directly correlates to on-target indel formation


Obtain results the same day without waiting for sequencing results or performing complicated sequencing analysis.For Research Use Only. Not for use in diagnostic procedures.


               Specifications


Format

Kit

No. of Reactions


20 Reactions

Polymerase

Taq Polymerase

Product Type


Genomic Cleavage Detection Kit

Quantity


20 reactions

Recognition Site


INDEL

Sufficient For


CRISPR-Cas9, TAL Effector Nuclease

Detection Method

Primer-probe

Form

Liquid

Reaction Speed

Fast

Unit Size

Each



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